Question 1

What is the primary stain used in the Gram stain?

Accepted Answer

A)   Crystal violet
B)   Safranin
C)   Malachite Green
D)   Methylene BlueE) B) and C)
F) A) and D)

Question 2

What is the incorrect match below?

Accepted Answer

A)   Light microscope/visible light and glass lenses.
B)   Electron microscope/electron beam and electromagnetic lenses.
C)   Nanoprobe microscope/ fine-tipped probes are used to penetrate the surface of a specimen to magnify internal structures.
D)   Confocal microscope/illuminates one focal plane at a time which is then reassembled into a three-dimensional image.E) A) and B)
F) B) and C)

Question 3

A bright field microscope ________. (Select all that apply)

Accepted Answer

A)   produces an image showing fluorescent portions of specimen
B)   is the simplest, most common type of microscope
C)   produces a bright image against dark background
D)   produces the image of a stained specimen is visualized against a bright backgroundE) A) and B)
F) A) and C)

Question 4

A dark field microscope ________.

Accepted Answer

A)   produces an image showing fluorescent portions of specimen
B)   is the simplest, most common type of microscope
C)   produces a bright image against dark background
D)   produces the image of a stained specimen is visualized against a bright backgroundE) A) and B)
F) None of the above

Question 5

The light micrograph below is an example of: ________.

Accepted Answer

A)   A capsule stain
B)   An acid fast stain
C)   An endospore stain
D)   Dark field microscopyE) A) and D)
F) None of the above

Question 6

A 20-year-old male presented at the emergency room with intense burning during urination. A copious amount of pus was being discharged from his penis. A micrograph of a gram stain of the pus is shown below. The man is infected with ________.

Accepted Answer

A)   gram negative bacteria that have infected leukocytes
B)   gram positive bacteria that have infected leukocytes
C)   gram positive bacteria and gram positive eukaryotic pathogens
D)   gram negative bacteria and gram negative eukaryotic pathogensE) A) and D)
F) A) and B)

Question 7

What two features of a microscope are essential for a microbiologist?

Accepted Answer

A)   Magnification and resolution
B)   Magnification and oil immersion
C)   Resolution and oil immersion
D)   Objectives and ocularsE) B) and D)
F) A) and C)

Question 8

The condenser ________.

Accepted Answer

A)   focuses light on specimen
B)   separately magnify specimen
C)   holds the specimen in place
D)   controls light intensity via adjustable openingE) All of the above
F) A) and C)

Question 9

The________ magnify the specimen on a light microscope.

Accepted Answer

A)   Iris diaphragm
B)   Condenser lens
C)   Objective and Ocular lenses
D)   StageE) A) and B)
F) All of the above

Question 10

Among the characteristics of Positive Staining are: ________. (Select all that apply)

Accepted Answer

A)   cells are colored against a clear background
B)   The positive charge of stain is attracted to negatively charges on DNA and surface proteins
C)   examples include nigrosin and India ink
D)   Examples include methylene blue and crystal violetE) A) and B)
F) A) and C)

Question 11

What is the primary stain used in the Acid-Fast stain?

Accepted Answer

A)   Crystal violet
B)   Carbol fuchsin
C)   Malachite Green
D)   Methylene BlueE) A) and B)
F) A) and C)

Question 12

Which of the following is a major characteristic of super-resolution microscopy? (Select all that apply)

Accepted Answer

A)   It increases the resolution of transmission electron microscopy
B)   It increases the resolution limit of a confocal microscope
C)   It is able to produce an image in very low light
D)   It can visualize living and dead cellsE) None of the above
F) B) and D)

Question 13

What type of microscope was used to produce the image below?

Accepted Answer

A)   Dark field microscope
B)   Bright field microscope
C)   Fluorescence microscope
D)   Phase contrast microscopeE) A) and D)
F) C) and D)

Exam 4: Microscopy

Which of the following is a major characteristic of super-resolution microscopy? (Select all that apply)

Correct Answer
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A bright field microscope ________. (Select all that apply)

Correct Answer
verified

A dark field microscope ________.

Correct Answer
verified

A 20-year-old male presented at the emergency room with intense burning during urination. A copious amount of pus was being discharged from his penis. A micrograph of a gram stain of the pus is shown below. The man is infected with ________.

Correct Answer
verified

The condenser ________.

Correct Answer
verified

What is the primary stain used in the Gram stain?

Correct Answer
verified

What type of microscope was used to produce the image below?

Correct Answer
verified

The________ magnify the specimen on a light microscope.

Correct Answer
verified

The light micrograph below is an example of: ________.

Correct Answer
verified

What two features of a microscope are essential for a microbiologist?

Correct Answer
verified

Among the characteristics of Positive Staining are: ________. (Select all that apply)

Correct Answer
verified

What is the primary stain used in the Acid-Fast stain?

Correct Answer
verified

What is the incorrect match below?

Correct Answer
verified

Exam 4: Microscopy

Which of the following is a major characteristic of super-resolution microscopy? (Select all that apply)

Correct AnswerverifiedShow Answer

A bright field microscope ________. (Select all that apply)

Correct AnswerverifiedShow Answer

A dark field microscope ________.

Correct AnswerverifiedShow Answer

A 20-year-old male presented at the emergency room with intense burning during urination. A copious amount of pus was being discharged from his penis. A micrograph of a gram stain of the pus is shown below. The man is infected with ________.

Correct AnswerverifiedShow Answer

The condenser ________.

Correct AnswerverifiedShow Answer

What is the primary stain used in the Gram stain?

Correct AnswerverifiedShow Answer

What type of microscope was used to produce the image below?

Correct AnswerverifiedShow Answer

The________ magnify the specimen on a light microscope.

Correct AnswerverifiedShow Answer

The light micrograph below is an example of: ________.

Correct AnswerverifiedShow Answer

What two features of a microscope are essential for a microbiologist?

Correct AnswerverifiedShow Answer

Among the characteristics of Positive Staining are: ________. (Select all that apply)

Correct AnswerverifiedShow Answer

What is the primary stain used in the Acid-Fast stain?

Correct AnswerverifiedShow Answer

What is the incorrect match below?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
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Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

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Correct Answer
verified

Correct Answer
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